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Due to behavioral differences in dogs, the visible signs of heat and breeding time (such as flagging, bleeding, or readiness to breed) are unreliable and only an approximate indication of the fertile period. Accurate ovulation timing is best done by measuring the hormone progesterone in the blood. Progesterone levels begin to rise 1-2 days before the LH (Luteinizing Hormone) peak, giving the earliest sign of ovulation thus providing both accuracy and convenience. This kit will enable the user to determine the optimum time for fertilization. Accurate timing is important for breeding success especially when the breeding is done with fresh chilled or frozen semen.
Test Preparation (For Serum or Plasma): Allow all kit components and samples to come to room temperature. Collect the blood sample into an EDTA or heparin coated tube (purple top tube) or in a dry tube (red top tube). Immediately after sample collection, invert the tube several times to mix. Spin the blood down with a centrifuge or allow the blood to clot by staying at room temperature for 1/2 to 1 hr and pour the serum into a clean glass tube. If the sample is not run immediately, store the sample in the refrigerator. For long term storage the sample should be frozen. Label the sample with the name, sample day and cycle day. Do not use whole blood. Do not run test with cold kits.
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