Target Canine Ovulation Kit for Dogs

Target Canine Ovulation Kit for Dogs
Kit of 6 Tests

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  • Kit of 6 Tests £111.04


Due to behavioral differences in dogs, the visible signs of heat and breeding time (such as flagging, bleeding, or readiness to breed) are unreliable and only an approximate indication of the fertile period. Accurate ovulation timing is best done by measuring the hormone progesterone in the blood. Progesterone levels begin to rise 1-2 days before the LH (Luteinizing Hormone) peak, giving the earliest sign of ovulation thus providing both accuracy and convenience. This kit will enable the user to determine the optimum time for fertilization. Accurate timing is important for breeding success especially when the breeding is done with fresh chilled or frozen semen.

Kit contents

  • 6 monoclonal antibody treated test cups
  • 6 sample applicators
  • 1 bottle of wash solution
  • 1 bottle of substrate buffer A
  • 1 bottle of substrate buffer B
  • 1 substrate mixing bottle
  • 1 bottle enzyme conjugate
  • A color chart

Test procedure

Test Preparation (For Serum or Plasma): Allow all kit components and samples to come to room temperature. Collect the blood sample into an EDTA or heparin coated tube (purple top tube) or in a dry tube (red top tube). Immediately after sample collection, invert the tube several times to mix. Spin the blood down with a centrifuge or allow the blood to clot by staying at room temperature for 1/2 to 1 hr and pour the serum into a clean glass tube. If the sample is not run immediately, store the sample in the refrigerator. For long term storage the sample should be frozen. Label the sample with the name, sample day and cycle day. Do not use whole blood. Do not run test with cold kits.

When to test

  1. One serum sample should be run during the first five days of the proestrus cycle (characterized by the presence of blood and vulval swelling) in order to determine the baseline progesterone level. The baseline progesterone level varies between 0-1 ng/mL in individual dogs and gives a bright blue color result similar to the C1 color on the color chart.
  2. Begin testing to determine the initial rise in progesterone about 11-13 days after observing the first day of vaginal bleeding and vulval swelling (the beginning of proestrus). If the first result is the baseline color, bright blue (C1), then the progesterone level is still very low.
  3. Retest every two days until the test results are a light blue that is similar to the C2 color on the color chart. This first fading of the color from C1 to C2 represents the initial rise in the progesterone level; ovulation will occur 1-2 days later.
  4. Retest every day until the test results are faint blue similar to the C3 color on the color chart. This means the egg is beginning to ripen and the fertile period begins 2-3 days later. After obtaining a C3 color, natural matings and inseminations with fresh semen should take place in 2 or 3 days and again in 2 days. If using frozen semen, inseminate in 2 or 3 days and every other day for a total of 3 times.
  5. If a white result is obtained, mating or insemination should be done immediately.

Need help or advice? Contact us:

  • Landline: 01582 842096
  • Mon - Fri: 8:00am - 5:45pm
  • Sat: 9:00am - 1:00pm
  • Email: [email protected]

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Q & A

Below are some recent questions we've received regarding Target Canine Ovulation Kit for Dogs, including answers from our team.

26 November 2021 at 10:43am

How do I collect blood sample?


Is there a syringe included in the kit to collect the blood sample?
Or do need to purchase separately?
Thank you,

  • VioVet Staff

This kit does not include equipment to sample the blood. This will need to be done by a trained professional, such as your local vet or vet nurse. We do supply syringes/needles on our site however if you are trained in venipuncture and need supplies.

12 October 2020 at 12:43pm

Re canine ovulation tests

jennifer semple

Can you please how accurate these tears are? For e.g compared to using the pads that are available.

Jennifer Semple

  • VioVet Staff

I have contacted Target, who manufacture this ovulation test, and they have responded with the following:

Target is very accurate, 95% accurate - when run at room
temperature according to directions.

If you need anything else then please contact us at [email protected].

18 August 2020 at 10:45am

Ovulation kit

Jacky Cheung Yue Man

How to do ovulation kit and can you shipped to China

  • VioVet Staff

These kits require blood samples in order to run, the full testing guidelines can be found in the description on the product page. Yes we can ship products to China. Please head to the checkout to see our most up to date delivery options and prices.

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